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The pellet was dissolved in sterile PBS solution and the bacterial density was measured at optical density (OD 600 ) and adjusted to 1.89 (1.8 10 9 cfu/ml approximately) and 10 ml of bacterial suspension was further used in bioassays as described by Eski et al

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Peer influence is rarely harnessed formally for prevention, though pupils often act independently to discourage peers from smoking

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